Ucwaningo lwethu lubonisa ukuthi i-Ganoderma lucidum/reishi/lingzhi ibonisa izakhiwo ze-antitumor kumaseli e-osteosarcoma ku-vitro.Kwatholakala ukuthi i-Ganoderma lucidum ivimbela ukukhula kwamangqamuzana omdlavuza webele kanye nokufuduka ngokucindezela ukubonakaliswa kwe-Wnt/β-catenin.Icindezela umdlavuza wamaphaphu ngokuphazanyiswa kokunamathela okugxilile kanye nokungeniswa kokucekelwa phansi kwe-MDM2-mediated Slug.I-Ganoderma lucidum ivimbela umdlavuza webele ngokunciphisa indlela ye-PI3K/AKT/mTOR, i-Ganoderma lucidum idlala indima elwa nesimila kumaseli e-acute leukemia ngokuvimba indlela ye-MAPK.
I-CCK-8 kanye nokuhlolwa kokwakhiwa kwekholoni, ukuhlola umphumela we-Ganoderma lucidum ekusebenzeni kwe-osteosarcoma cell line nokwanda, kubonise ukuthi i-Ganoderma lucidum icindezela ukwanda kwamaseli e-MG63 kanye ne-U2-OS ngendlela encike esikhathini kanye nokugxila, futhi inciphisa ikhono lamaseli ukuba enze ikoloni.
I-Ganoderma lucidum ilawula ukuvezwa kwezakhi zofuzo ze-proapoptotic, futhi ukuhlaziywa kwe-cytometry yokugeleza kubonise ukuthi i-apoptosis ye-MG63 kanye namaseli e-U2-OS yanda ngemva kokwelashwa nge-Ganoderma lucidum.Ukufuduka kwamangqamuzana kuyisisekelo sokuziphatha okuhlukahlukene kwebhayoloji, okuhlanganisa i-angiogenesis, ukuphulukiswa kwesilonda, ukuvuvukala, kanye ne-metastasis yomdlavuza.I-Ganoderma lucidum inciphisa ukufuduka nokuhlasela kwayo yomibili imigqa yamaseli futhi ivimbele ukwanda, ukufuduka, nokuhlasela, futhi ibangele i-apoptosis yamaseli e-osteosarcoma.
Ukusayina kwe-Aberrant Wnt/β-catenin kuhlobene eduze nokwakheka, i-metastasis, kanye ne-apoptosis yezinhlobo eziningi zomdlavuza, ngokukhushulwa kokusayina kwe-Wnt/β-catenin okubonwa ku-osteosarcoma.
Kulolu cwaningo, ukuhlolwa kwentatheli ye-dual-luciferase kubonise ukuthi ukwelashwa kwe-Ganoderma lucidum kuvimbela ukusayinwa kwe-CHIR-99021 okucushwe i-Wnt/β-catenin.Lokhu kufakazelwa futhi ukuboniswa kwethu ukuthi ukulotshwa kwezakhi zofuzo eziqondiswe ku-Wnt, njenge-LRP5, β-catenin, cyclin D1, ne-MMP-9, kuyavinjelwa lapho amaseli e-osteosarcoma ephathwa nge-Ganoderma lucidum.
Ucwaningo lwangaphambilini lubonise kumasampula omtholampilo ukuthi i-LRP5 ilawulwa kahle ku-osteosarcoma uma kuqhathaniswa nezicubu ezijwayelekile, kanye nokubonakaliswa kwe-LRP5 kuhlobana nesifo se-metastatic kanye nempilo embi engenazifo, okwenza i-LRP5 ibe inhloso yokwelapha engaba khona ye-osteosarcoma.
I-β-catenin ngokwayo iyinhloso eyinhloko endleleni yokubonisa i-Wnt/β-catenin, futhi inkulumo ye-β-catenin ku-osteosarcoma yanda kakhulu.Lapho i-β-catenin ithuthela ku-nucleus isuka ku-cytoplasm, yenza kusebenze ukuvezwa kwezakhi zofuzo eziqondiswe ezansi nomfula, ezihlanganisa i-cyclin D1, C-Myc, kanye nama-MMP.
I-Myc ingenye yama-proto-oncogenes amakhulu futhi idlala indima ebalulekile ekulawuleni ukwenziwa kusebenze, ukulotshwa, kanye nokuvinjelwa kokuvezwa kofuzo.Kubikwe ukuthi ukucindezelwa kwe-C-Myc oncogene kubangela ukuguga kanye ne-apoptosis yezinhlobo ezimbalwa zamaseli wesimila, okuhlanganisa i-osteosarcoma.
I-Cyclin D1 iyisilawuli sesigaba samaseli esibalulekile se-G1 futhi isheshisa ukuguqulwa kwesigaba se-G1/S.Ukubonakaliswa okweqile kwe-cyclin D1 kunganciphisa umjikelezo weseli futhi kukhuthaze ukwanda okusheshayo kwamaseli ezinhlotsheni ezihlukene zesimila.
I-MMP-2 ne-MMP-9 ama-stromelysins anamandla okwehlisa izinga lezingxenye ze-matrix ezingaphandle kwamaseli, isici esibalulekile se-tumor angiogenesis nokuhlasela.
Lokhu kuphakamisa ukuthi izakhi zofuzo ezihlosiwe ze-Wnt/β-catenin zidlala indima ebalulekile ekuqhubekeleni phambili kwe-osteosarcoma, nokuthi ukuvimba lawa ma-node esignali kungase kube nomphumela omangalisayo wokwelapha.
Ngokulandelayo, sithole ukuvezwa kwe-mRNA kanye nephrotheni ye-Wnt/β-catenin yofuzo oluqondiwe oluhlobene nokusayina nge-PCR kanye ne-western blotting.Kuyo yomibili imigqa yamaseli, i-Ganoderma lucidum ivimbele ukuvezwa kwalawa maprotheni nezakhi zofuzo.Le miphumela iqhubeka ibonisa ukuthi i-Ganoderma lucidum ivimbela ukusayinda kwe-Wnt/β-catenin ngokukhomba i-LRP5, i-β-catenin, i-C-Myc, i-cyclin D1, i-MMP-2, ne-MMP-9.
I-E-cadherin iyi-transmembrane glycoprotein evezwa kabanzi kumaseli e-epithelial futhi ilamula ukunamathela phakathi kwamaseli e-epithelial namaseli e-stromal.Ukususwa noma ukulahleka kwesisho se-E-cadherin kuholela ekulahlekelweni noma ekwehleni kokunamathela phakathi kwamaseli wesimila, okuvumela amaseli wesimila ukuthi anyakaze kalula, bese kwenze isimila singene, sihlakazeke, futhi simetastaze.Kulolu cwaningo, sithole ukuthi i-Ganoderma lucidum ingakwazi ukulawula i-E-cadherin, ngaleyo ndlela iphikisana ne-Wnt/β-catenin-mediated phenotype yamaseli e-osteosarcoma.
Sengiphetha, imiphumela yethu ikhombisa ukuthi i-Ganoderma lucidum ivimba ukusayina kwe-osteosarcoma Wnt/β-catenin futhi ekugcineni iholele ekwehleni komsebenzi weseli le-osteosarcoma.Lokhu okutholakele kusikisela ukuthi i-Ganoderma lucidum ingaba i-ejenti yokwelapha ewusizo futhi esebenzayo yokwelapha i-osteosarcoma, imikhiqizo ehlobene ihlanganisa.i-ganoderma lucidum spore oil softgels/reishi spore oil ama-softgels,i-ganoderma lucidum spore powder/reishi spore powder
Isikhathi sokuthumela: Apr-18-2022